Short-chain fatty acids quantitative determination methods in Arctium Lappa L. leaves biomass extracts
- Authors: Khaibullin R.G.1, Tumilovich E.Y.2, Volkova L.V.1, Malkova T.L.2
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Affiliations:
- Perm National Research Polytechnic University
- Perm State Pharmaceutical Academy
- Issue: Vol 25, No 11 (2022)
- Pages: 16-22
- Section: Articles
- URL: https://journals.eco-vector.com/1560-9596/article/view/113005
- DOI: https://doi.org/10.29296/25877313-2022-11-03
- ID: 113005
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Abstract
Relevance. Fermentation of Arctium lappa L. (burdock) leaves with the Lactobacillus plantarum microorganisms makes it possible to obtain biologically active substances containing a range of compounds in its composition. These compounds determined by the used plant material composition and synthesized bacterial metabolites, one of which are short-chain fatty acids (SCFA) that play important role in maintaining human health. In combination with the polysaccharide inulin, present in the leaves of A. lappa L, SCFA in the fermented product will have a complex effect, including prebiotic and metabiotic activity. This fermented biomass can be used as a pharmaceutical substance in a drugs production for the prevention and concomitant therapy of metabolic disorders.
Aim of the research - Development of SCFAs determination methods in A. lappa L. leaf biomass samples aqueous extracts. Evaluation of the using microbial fermentation effectiveness to obtain biologically active substances.
Material and methods. The objects of study - aqueous extracts (1:10) of A. lappa L. leaf biomass samples, obtained by drying, traditional fermentation, and microbial fermentation by L. plantarum 8P-A3 microorganisms. Research methods - gas-liquid and high-performance liquid chromatography
Results. As a result, a method of some SCFA determination in plant extracts was developed. It has been established, that the acids-metabolites content in microbial-fermented A. lappa L. leaves biomass sample is higher than the acids content in samples obtained by drying and traditional fermentation.
Conclusion. The developed conditions of acetic, butyric and lactic acids determination in plant extracts by gas-liquid and high-performance liquid chromatography can be used to analyze analogous products, as well as to develop determining the entire spectrum of SCFA methods. Plant raw materials fermentation by L. plantarum microorganisms makes it possible to obtain SCFA-enriched substances, which can be used to produce therapeutic and prophylactic drugs.
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About the authors
R. G. Khaibullin
Perm National Research Polytechnic University
Author for correspondence.
Email: ruslan.khaibullin@yandex.ru
Post-graduate Student, Department of Chemistry and Biotechnology
Russian Federation, PermE. Y. Tumilovich
Perm State Pharmaceutical Academy
Email: e.tumilovich@gmail.com
Ph.D. (Pharm.), Associate Professor, Department of Toxicological Chemistry
Russian Federation, PermL. V. Volkova
Perm National Research Polytechnic University
Email: wolkowalw@mail.ru
Dr.Sc. (Med.), Professor, Department of Chemistry and Biotechnology
Russian Federation, PermT. L. Malkova
Perm State Pharmaceutical Academy
Email: malkova-tl@pfa.ru
Dr.Sc. (Pharm.), Professor, Department of Toxicological Chemistry
Russian Federation, PermReferences
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